Lab Report Osmosis

The effect of osmosis on schmaltzy jail carrelular telephoneular phoneular phones with assorted concentrations of saccharose Alex McRae biota 120-902 constant of gravitation Valley State University 1 Campus study aloneendale, MI 49401 emailprotected gvsu. edu Abstract In this study, we tried the validity of osmosis in fake animal cells. Osmosis is the diffusion of free pee crosswise a membrane. The purpose of the study was to omen the assess of osmosis in artificial cells containing several(predicate) concentrations of sucrose and irrigate.We study the esteem of osmosis in artificial cells by creating five different dialysis old foundations with different concentrations of both sucrose and irrigate supply and calculating the acacac cumulative counter channelize in taket unit ever 10 transactions for 90 minutes. Our results for the artificial cells showed different concentrations go from uplifted to first base concentrations- through hypotonic huntin g expedition or hypertonic movement. installation The main purpose of this paper is to appreciate the rank of counter alter with osmosis for different concentrations of sucrose in artificial cells.Since the human torso is composed of trillions of cells that contain roughly 85% of peeing, makes osmosis a very important fantasy (Carmichael, Grabe and Wenger). The forces that affect osmosis be the concentrations of solutes surrounding the cell or inwardly of the cell. Water entrust then move across the cell membrane and create a isotropy of water between the cell and its purlieu (Reece et al. 133).In order to calculate the average pace of change for our artificial cells, we essential fancy tonicity as the ability of a nearby solution to cause a cell to lose or acquire water, depending on its concentration of non-pe earnrating solutes relative to solutes in spite of appearance the cell (Reece et al. 133). The dialysis al-Qaidas used in this turf out have membranes wh ich are selectively permeable, which provided relinquishs particles specifically small enough to afford through (Carmichael, Grabe and Wenger).In a hypotonic solution, water goes into the cell because the solute is to a greater extent concent evaluated inside the cell, while in a hypertonic solution, water moves out of the sell because the solute is more than concentrated outside of the cell. We are test the effect of osmosis on different concentrations of artificial cells by calculating the cumulative change in incubus unit and the corrected cumulative changes in weight and by muster out whether a solution is hypertonic, hypotonic or isosmotic. We predicted that a dialysis cornerstone guardianship bump water in a beaker excessively containing strike hard water is in an isosmotic solution.While 20% sucrose, 40% sucrose and 60% sucrose in beakers containing whang water is considered hypotonic solutions. Lastly the dialysis stem holding dab water in a beaker contain ing 40% sucrose is a hypertonic solution. This give result in isotonic solutions remaining at the same weight, hypotonic solutions gaining weight and hypertonic solutions losing weight. We tried this by creating the five different dialysis suitcases with different concentrations of sucrose in order to measure the weight change in grams of the bag afterward nine 10 minute increments. Methods and MaterialsThis test took place on Monday, February 6th, 2011. During this fourth dimension, we tested the do of different sucrose concentrations on the rate of osmosis in artificial cells we made with dialysis tube. We studied five different dialysis bags containing 10mL of different concentrations of tap water and sucrose. Two contained tap water while three contained different concentrations of sucrose, vary from 20% to 60%. Each bag was placed in a beaker ring by either tap water or 40% sucrose. We began the sample by soaking the dialysis tubes to prepare them for the sucrose con centrations they would be make full with.Taking individually bag, two were filled with 10mL of tap water, one filled with 10mL of 20% sucrose, one with 10mL of 40% sucrose and other with 10mL of 60% sucrose. Each bag was clamped closed. All the bags were weighed before being placed in their corresponding beakers in order to picture their initial weight in grams. The bags were rate in their corresponding beakers, all of which contained tap water, except beaker 5 (tap water bag 5 was placed in beaker 5 which instead of holding water, was filled with 40% sucrose) concurrently, recording the time.In the same manner in which the bags were placed in the beakers simultaneously, remove the bags all 10 minutes, and record the weight of for severally one bag. This process should be repeated for at least 90 minutes total. This data was analyzed by calculating the cumulative change in weight for to to each one one dialysis bag. This was done from subtracting the weight of each bag fr om the initial weight of the bag. Doing so, allows the weight of each bag to be initially zero. For that, we must calculate the corrected cumulative change in weight.For each time separation of 10 minutes, we subtracted the change in weigh of bag 1 (tap water) from the weight of each bag at the specific time measure- this corrected any oscillations. Results The corrected cumulative change in weight referable to osmosis from different concentrations of sucrose and tap water, are shown in Figure One. This figure shows the weight change in grams for every time interval of 10 minutes. victimization the corrected cumulative change in weight eliminates bag 1 because its average rate of change will always be zero.Below is a table of the bag weights at 10 minute intervals after being tested for an hour suitcase Weights (g) Time (min) 1 2 3 4 5 Water 20% 40% 60% water 0 21. 81 20. 30 23. 3 21. 30 19. 22 10 22. 75 26. 94 22. 04 23. 64 18. 42 20 22. 29 26. 91 22. 29 24. 41 17. 95 30 23. 27 29. 33 23. 45 26. 41 16. 60 40 22. 30 29. 84 23. 24 28. 6 15. 61 50 22. 72 36. 63 24. 02 28. 84 14. 75 60 23. 29 31. 20 24. 51 30. 17 14. 05 The purpose of this experiment was to determine the consanguinity between concentration gradients and the rates of osmosis. Using the corrected cumulative change, we can monitor lizard the rate of change for each bag, and agree the rate of change to the rate of osmosis.For bag 2, the slope, or the rate of osmosis was y = 0. 1193x 1. 7293, displaying a slow but obvious attach in weight, or a hypotonic solution, when the solute was more concentrated inside the cell and water travel into the cell. Bag 3 continues to show this trend with a speedy rate of y = 1. 295x 2. 4807, which water enters this bag as a hypotonic solution. Bag 4, which a rate of y = -1. 0586x + 1. 9043, shows a hypertonic solution in which the low concentration solute, causing water inside the dialysis bag, to move out.Although it was expected for for bag 5 , which was tap water submersed in 40% sucrose, to be hypertonic, the rate of osmosis was y = 1. 3536x 0. 1679, which shews a hypotonic solution, or water go in the cell, or piteous from a high concentration of the solute to a low concentration. These results prove that the focus of osmosis does directly affect the rate of osmosis. If the slope begins with a negative x value, the solution is indeed a hypertonic solution, that when surrounding a cell will cause the cell to lose water, moving from a high concentration to a lower concentration (Reece et al. 33). The slopes which begin with a positive x value demonstrate a hypotonic solution, which causes a cell to take in water (Reece et al. 133). This shows that the solicitude of osmosis is directed related to the rate of osmosis, or vice versa. The rate of osmosis ultimately determines the direction of osmosis. Depending on which direction osmosis is going- hypertonic, isotonic or hypotonic, determines the rate of osmosis, or t he rate of change for each dialysis bag. Or by the factor of our experiment, the direction of osmosis was determined by the rate of change in each bag, or the rate of osmosis.Discussion Throughout the study it was cogitate that different concentrations of sucrose are allow different rates and directions of osmosis. The results show that the rate of osmosis is directly related to the direction of osmosis, or vice versa. This proposal does not encounter with our quantitative prediction. Our results for the artificial cells showed different concentrations moved from high to low concentrations- through hypotonic movement or hypertonic movement however bag 3 with 40% sucrose was expected to be a hypotonic solution, while it was a hypertonic solution.This falsified hypothesis could be due to the score that in an animal cell, when a hypertonic solution, the cell experiences crenation. The dialysis tubing creates a hypothetical flaw in our experiment because the tubing has a molecular weight brush off off of a maximum of 14 kilodaltons, while the average human cell may have a bigger or smaller molecular weight cut off, allowing the cell to experience different tonicities. In order to obtain more consummate results, modifications should be made. More drastic concentrations of sucrose in the dialysis tubing should be tested in order to find the extremes of the rate of change for osmosis.The study enhances the infix scholarship in this area by exposing osmosis along a free heartiness gradient. However, other experiments could increase our knowledge almost the relationship between concentration gradients and rates. An experiment that includes the idea that the selectively permeable membrane moves, might allow for more accurate results (Patlak and Watters). The qualified location mirrors the volume of each side of the membrane, which affects the total number of particles on each side (Patlak and Watters).Our experiment exposes the ensample notion that there is n o net movement of a solvent and the water is what diffuses across the membrane. Works Cited Carmichael, Jeff, Mark Grabe and Jonathan Wenger. Biology 150 Laboratory Review. University of North Dakota, n. d. Web. 7 Oct. 2011. Patlak, Joseph and Chris Watters. Diffusion and Osmosis. University of Vermont and Middlebury College, 1997. Web. 8 Oct. 2011. Reece, Jane B. , et al. Campbell Biology. San Francisco Pearson Education Inc. , 2005. Print.